饲料添加剂类抗生素替代品在生猪养殖中的研究进展

随着现代社会的发展,集约化养殖成为主流趋势,抗生素的缺失往往会给养殖者带来巨大损失。但是,长期以来,抗生素滥用导致的细菌耐药性和药物残留问题日益严峻。随着我国2020年饲料添加剂类的抗生素的禁用,寻找高速、有效的抗生素替代品成为养殖业急需解决的重要问题。近年来,涌现出的各种饲料添加剂,如酸化剂、微生态制剂、酶制剂、植物提取物、抗菌肽等均表现出良好的杀菌、抑菌、促进动物生长、提高免疫力的效果,为抗生素替代提供了解决思路。文章对近年来一些抗生素替代品在生猪养殖方面的研究应用情况进行了综述,为替抗用饲料添加剂使用提供参考。     

硫化细菌的分离及对碱土pH的影响

通过对样品的富集，利用选择培养进行化能自养硫化细菌的分离；以大庆本地碱土作为实验材料，通过硫化细菌及辅料的添加，利用pH计测定碱土pH值变化。分离获得了硫化细菌lh-1，利用该菌株改良碱土以添加0.27% 硫粉混合硫化细菌效果最为明显，经过两个半月的改良，pH可从10.53降到8.23。     

土壤调理粉剂处理下茶园土壤重金属污染特征及潜在风险评价

本文以贵州安顺不同土壤调理粉剂处理下“瀑布毛峰”茶园土壤为供试样品,通过样品测试与理化分析,对CK处理(0 kg/hm²)、A处理(600 kg/hm²)、B处理(750 kg/hm²)和C处理(900 kg/hm²)下茶园土壤重金属含量特征及污染水平进行探讨。结果表明：不同土壤调理粉剂处理下,研究区域土壤重金属Cr、Hg、Pb、Cu、Zn、Ni、As和Cd的变异程度各异,且土壤中这8种重金属含量也各不相同,其中pH、Cr、Pb和Ni含量在C处理中最高,As、Hg和Cd含量在A处理中最高,Cu和Zn含量在B处理中最高;对各重金属元素进行相关性分析可知,Ni与Pb之间存在极显著相关性,表明两者之间的同源性极高,其余元素之间相关性特征不明显;由综合污染指数（P_综）得出A处理为轻度污染,CK处理、B处理和C处理均为中度污染,不同处理土壤中Cr均在国家Ⅱ级标准(GB15618—2008)规定范围内,样点达标率为100%,在所选定的污染评价因子中,Cu的贡献率最高,为土壤主要影响因子,其次是Cd和Ni;研究区土壤重金属单项潜在生态风险指数($E_{r}^{i}$)中Hg属于强生态风险,其余7种重金属处于轻微潜在生态风险等级;综合潜在生态风险指数(RI)平均值为196.79,总体处于中等潜在生态风险水平,Hg对RI的贡献率最大,是最主要的生态风险元素。     

土壤管理措施对坡耕地侵蚀退化耕层的恢复作用

【目的】紫色土坡耕地土壤侵蚀严重,研究土壤管理措施对不同侵蚀程度坡耕地耕层侵蚀恢复作用,为紫色土坡耕地耕层质量调控和坡耕地持续利用提供理论及实践依据。【方法】采用单因素方差分析（One-way ANOVA）检验各指标的差异显著性,研究不施肥（CK）、施化肥（F）、施生物炭+化肥（BF）3种土壤管理措施对侵蚀0 cm（S_-0）、侵蚀5 cm（S_-5）、侵蚀10 cm（S_-10）、侵蚀15 cm（S_-15）、侵蚀20 cm（S_-20） 5个不同侵蚀程度坡耕地耕层土壤属性的恢复作用。【结果】（1）随侵蚀程度增加,土壤砂粒含量由38.1%—42.4%逐渐增至44.2%—46.4%,土壤黏粒含量由12.6%—14.8%逐渐减少至9.6%—11.0%;与S_-0、S_-5、S_-10相比,S_-15、S_-20土壤容重显著增大;S_-10侵蚀程度下,土壤抗剪强度最大,在8.71—9.56 kg·cm^-2之间;F、BF处理下S_-15侵蚀程度的土壤稳定入渗率、平均入渗率降幅均最大。（2）不同管理措施下坡耕地耕层土壤属性差异显著,BF处理下土壤砂粒含量小、黏粒含量最大,0—10 cm、10—20 cm土层土壤容重显著低于CK（P<0.05）,土壤总孔隙度、毛管孔隙度均显著高于CK、F处理;BF处理下土壤初始入渗率、稳定入渗率、平均入渗率均最大,CK处理最小;与CK处理相比,BF处理土壤抗剪强度显著增大。（3）随侵蚀程度增加,土壤可蚀性K值显著下降,与S_-0相比,S_-20侵蚀程度下土壤可蚀性K值下降0.1960%—0.2192%;BF处理下土壤可蚀性K值最大,F处理次之,CK最小;S_-10侵蚀程度下F、BF处理的K值均较CK增加幅度最大,分别增加0.0684%、0.1404%。【结论】施加生物炭+化肥在改善土壤物质组成、结构特征、提高土壤蓄渗性能等方面较单施化肥效果更为显著,可有效减轻紫色土坡耕地耕层土壤侵蚀,对侵蚀10 cm条件下的坡耕地耕作层（0—20 cm）土壤的改良效果最佳。     

蚯蚓养殖对幼龄胶园土壤养分及橡胶树根系生长的影响

本研究旨在探讨林下蚯蚓养殖对幼龄胶园土壤养分及橡胶树根系生长的影响。以牛粪作为原料，于 2017年4—11 月在 4 年生幼龄胶园铺设粪垄养殖蚯蚓，并测定土壤养分和根系生长情况。结果表明，与对照（CK）相比，铺设粪垄无蚯蚓养殖（WQ）和铺设粪垄蚯蚓养殖（Q）处理总体上可显著提高土壤硝态氮、速效磷、速效钾和土壤水分含量以及土壤 pH，而对土壤铵态氮、有机质、微生物活性及微生物群系数量无显著影响（p<0.05）；和 CK 相比，WQ和 Q 处理显著促进根的生长，并提高吸收根的比例（p<0.05）。综上所述，在幼龄胶园养殖蚯蚓短期内（半年）可提高土壤速效养分含量，促进根系生长。     

Cloning and expression analysis of innate immune genes from red sea bream to assess different susceptibility to megalocytivirus infection

As suggested by the Office International des Epizooties (OIE), fishes belonging to the genus Oplegnathus are more sensitive to megalocytivirus infection than other fish species including red sea bream (Pagrus major). To assess the roles of the innate immune response to these different susceptibilities, we cloned the genes encoding inflammatory factors including IL‐8 and COX‐2, and the antiviral factor like Mx from red sea bream for the first time and performed phylogenetic and structural analysis. Analysed expression levels of IL‐1β, IL‐8 and COX‐2 and the antiviral factor like Mx genes performed with in vivo challenge experiment showed no difference in inflammatory gene expression or respiratory burst activity between red sea bream and rock bream (Oplegnathus fasciatus). However, the Mx gene expression levels in red sea bream were markedly higher than those in rock bream, suggesting the importance of type I interferon (IFN)‐induced proteins, particularly Mx, during megalocytivirus infection, rather than inflammation‐related genes. The in vitro challenge experiments using embryonic primary cultures derived from both fish species showed no difference in cytopathic effects (CPE), viral replication profiles, and inflammatory and Mx gene expression pattern between the two fish species.     

terraGIS – a web GIS for delivery of digital soil maps in cotton‐growing areas of Australia

Effective management of soil requires the spatial distribution of its various physical, chemical and hydrological properties. This is because properties, for example clay content, determine the ability of soil to hold cations and retain water. However, data acquisition is labour intensive and time‐consuming. To add value to the limited soil data, remote sensing (e.g. airborne gamma‐ray spectrometry) and proximal sensing, such as electromagnetic (EM) induction, are being used as ancillary data. Here, we provide examples of developing Digital Soil Maps (DSM) of soil physical, chemical and hydrological properties, for seven cotton‐growing areas of southeastern Australia, by coupling soil data with remote and proximal sensed ancillary data. A greater challenge is how to get these DSM to a stakeholder in a way that is useful for practical soil use and management. This study describes how we facilitate access to the DSMs, using a simple‐to‐use web GIS platform, called terraGIS. The platform is underpinned by Google Maps API, which is an open‐source development environment for building spatially enabled Internet applications. In conclusion, we consider that terraGIS and the supporting information, available on the sister web page ( http://www.terragis.bees.unsw.edu.au/ ), allow easy access to explanation of DSM of soil properties, which are relevant to cotton growers, farm managers, consultants, extension staff, researchers, state and federal government agency personnel and policy analysts. Future work should be aimed at developing error budget maps to identify where additional soil and/or ancillary data is required to improve the accuracy of the DSMs.     

熟石灰施用对土壤pH及附子生长发育的影响

[目的]明确熟石灰对附子地酸性土壤的改良作用以及对附子生长发育的影响。[方法]开展盆栽试验研究熟石灰不同施用量对土壤pH、附子生长发育及产量的影响。[结果]不同熟石灰处理(1 350、1 800、2 250 kg/hm~2)均可显著提高土壤pH(P0.05),且不同程度地促进附子出苗、抽茎;中、低用量熟石灰(1 350、1 800 kg/hm~2)能促进附子株高,而高用量(2 250 kg/hm~2)则抑制植株生长;所有处理对附子苗期展叶数均无显著影响;熟石灰用量为1 800 kg/hm~2能提高单株附子产量,而用量为2 250 kg/hm~2能提高附子成活率,促进附子总产量提高。[结论]一定用量的熟石灰能有效调节酸性土壤pH,促进附子生长发育并提高产量。     

Growth and yield responses of upland NERICAs to variable water management under field conditions

This study aimed to investigate the possible causes for inconsistent performances of upland New Rice for Africa (NERICA) varieties in uplands and lowlands, while identifying important determinants in grain yield under deficient soil moisture. We compared the growth and yield of NERICA 1 and NERICA 5 to those of Yumenohatamochi, a Japanese upland variety, and Hinohikari, a Japanese lowland variety, subjected to different water management regimes (continually flooded, supplementary irrigation, and non-irrigation). Under conditions of deficient soil moisture, panicle number per square meter, spikelet number per panicle, and 1000-grain weight of NERICAs decreased, whereas the panicle number of the Japanese varieties experienced little change. In contrast, the grain filling ratio was unaffected by water management, irrespective of variety. The primary source of yield reduction under low soil water conditions was a decrease in spikelet number per panicle, and water stress intensity was the primary factor for the degree of this reduction. Variation in the abortion of secondary rachis-branches caused differences between NERICAs in their spikelet number response to soil moisture deficiency. The inconsistency in NERICA performance across uplands vs. lowlands can be partially attributed to variation in yield response to low soil water conditions. Moreover, water stress intensity and the presence of a water gradient along the vertical soil profile may combine to affect the fluctuation in NERICA performance under upland conditions.     

Effects of dietary protein and lipid levels with different protein‐to‐energy ratios on growth performance,feed utilization and body composition of juvenile red‐spotted grouper,Epinephelus akaara

An 8‐week feeding trial was conducted to assess dietary protein and lipid levels on growth performance, feed utilization and body composition of juvenile red‐spotted grouper (7.85 ± 0.03 g fish^?1). Nine semi‐purified diets were formulated containing varying protein levels (440–520 g kg^?1, dry matter) and lipid levels (60–120 g kg^?1, dry matter). The weight gain of juvenile Epinephelus akaara was affected by dietary protein (p = .005) and its interaction with dietary lipid (p = .020). Viscerosomatic index, intraperitoneal fat ratio and whole‐body lipid level increased with increasing dietary lipid level (p < .001). Nitrogen retention was not affected by dietary protein and lipid, while lipid retention decreased with increasing dietary lipid level (p < .001). The plasma blood urea nitrogen increased with increasing dietary protein level (p = .003). This study showed that diet with 520 g kg^?1 protein and 60 g kg^?1 lipid with 30.58 mg kJ^?1 P:E provided a maximal growth for this species. Moreover, an increase in dietary lipid levels (from 60 to 90 g kg^?1) could reduce the protein requirement (from 520 to 480 g kg^?1) without affecting the growth performance, while higher fat deposition was observed in fish fed high‐lipid diets.